8/16/2023 0 Comments Spore test![]() ![]() ![]() When decolorized with acid alcohol, color washes off the vegetative cells, making them colorless. Principle of Dorner’s method for staining endosporesĬarbol fuchsin, when applied to a heat-fixed slide and heated, softens the structure of the bacterial spores and the basic fuchsin gets into the spores. It employed a lengthy heating step but resulted in differential staining of endospores and vegetative cells in the same sample. This method utilizes carbol fuchsin as primary stain, acid alcohol as decolorizer, and nigrosin as counterstain. Dorner’s Methodĭorner’s method is an alternative method for staining the endospores published by Dorner in 1922. Observe the bacteria under 1000X (oil immersion) total magnification. When viewed under 1000X of a light microscope, vegetative cells appear pink/red, and spores appear green. Rinse both sides of the slide to remove the secondary stain and blot the slide/ air dry. ![]()
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